Topbp1 foci

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August undefined, 2024

Web2. aug 2024 · The role of TOPBP1 goes beyond the typical replication stress response; it interacts with the low-complexity nucleolar protein Treacle (also referred to as TCOF1) and forms large Treacle-TOPBP1 foci inside the nucleolus. Web12. jan 2004 · TopBP1 foci are abundant during early prophase I and localize mainly to histone γ-H2AX–positive domains, where DNA double–strand breaks (required to initiate recombination) occur. Strikingly, TopBP1 showed a pattern almost identical to that of ATR, a PI3K-like kinase involved in mitotic DNA damage checkpoint. ...

Structural Insights into Recognition of MDC1 by TopBP1 …

Web( Dc ) TopBP1 foci are seen in an MRL dermal cell nucleus. Source publication Lack of p21 expression links cell cycle control and appendage regeneration in mice [Cell_Biology] Web10. aug 2001 · Inhibition of DNA synthesis leads to relocalization of TopBP1 together with Brca1 to replication forks, suggesting a role in rescue of stalled forks. DNA damage induces formation of distinct TopBP1 foci that colocalize with Brca1 in S phase, but not in G (1) phase. We also show that TopBP1 interacts with the checkpoint protein hRad9. hello kitty tea party

TopBP1 deficiency impairs the localization of proteins involved in ...

Web17. aug 2015 · Persistent TopBP1 foci transition into 53BP1 nuclear bodies (NBs) in G1 and precise temporal depletion of TopBP1 just before mitotic entry induced formation of … Web28. máj 2024 · BRCT domains of TOPBP1 selectively bind conserved phosphorylation sites in the N-terminus of 53BP1. Mutation of these sites does not affect formation of 53BP1 or ATM foci following DNA damage, but abolishes recruitment of TOPBP1, ATR and CHK1 to 53BP1 damage foci, abrogating cell cycle arrest and permitting progression into S-phase. Web1. feb 2016 · Topoisomerase IIβ-binding protein 1 (TOPBP1) participates in DNA replication and DNA damage response; however, its role in DNA repair and relevance for human … hello kitty team usa funko

TopBP1 assembles nuclear condensates to switch on ATR

Web12. jan 2004 · Mammalian TopBP1 is a BRCT domain–containing protein whose function in mitotic cells is linked to replication and DNA damage checkpoint. Here, we study its possible role during meiosis in mice. TopBP1 foci are abundant during early prophase I and localize mainly to histone γ-H2AX–positive domains, where DNA double–strand breaks (required … WebLike γH2AX, TopBP1 foci were markedly enriched in the healer cells, indicative of an active and constitutive DNA damage checkpoint (Fig. 3D). damage, we performed a comet assay at both neutral ... hello kitty tea kettleWebThe results showed that HTATSF1 formed discernable foci co-localizing with mCherry-LacI-TOPBP1 (Figure S1E), whereas mutation of K155, K250, or in combination significantly impaired the redistribution of HTATSF1 to variable extents: double mutation abolished HTATSF1 foci formation and K250A had a much stronger effect than K155A (Figure 1E ... hello kitty tea drops

"Web24. sep 2010 · TopBP1 is a checkpoint protein that colocalizes with ATR at sites of DNA replication stress. In this study, we show that TopBP1 also colocalizes with 53BP1 at sites of DNA double-strand breaks (DSBs), but … " - Topbp1 foci

Topbp1 foci

Phosphorylation-mediated interactions with TOPBP1 couple …

Web6. aug 2013 · Activation of the DNA replication checkpoint by the ATR kinase requires protein interactions mediated by the ATR-activating protein, TopBP1. Accumulation of … Web26. jan 2024 · We induced TopBP1 condensation during the CldU pulse using two cycles of 3 min blue light illumination, in order to actuate and maintain TopBP1 foci during the 20 min labeling period (Figure 5E). DNA replication tracks labeled in the presence of optogenetic TopBP1 condensates were shorter than DNA replication tracks labeled in the absence of ...

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Web1. jan 2002 · We report here that TopBP1 is phosphorylated in response to DNA double-strand breaks and replication blocks. TopBP1 forms nuclear foci and localizes to the sites of DNA damage or the arrested replication forks. In response to DNA strand breaks, TopBP1 phosphorylation depends on the ataxia telangiectasia mutated protein (ATM) in vivo. Web28. máj 2024 · BRCT domains of TOPBP1 selectively bind conserved phosphorylation sites in the N-terminus of 53BP1. Mutation of these sites does not affect formation of 53BP1 …

WebWe report here that TopBP1 is phosphorylated in response to DNA double-strand breaks and replication blocks. TopBP1 forms nuclear foci and localizes to the sites of DNA damage … Web18. jan 2024 · It was reported that 53BP1 spontaneous foci were mainly presented in G phase cells and will be resolved during late G1 to S phase through DSB repair …

Web10. aug 2001 · Topoisomerase IIbeta-binding protein (TopBP1), a human protein with eight BRCT domains, is similar to Saccharomyces cerevisiae Dpb11 and Schizosaccharomyces … Web27. apr 2015 · To study the possibility that nucleolar eGFP-TopBP1 foci represent damaged DNA, we immunostained cells expressing eGFP-TopBP1 with anti-γH2AX and anti-53BP1, two commonly used markers for DNA double strand breaks and other DNA damage types. Neither of the two DNA damage marker proteins co-localized with the nucleolar eGFP …

Web25. jan 2016 · We show that TOPBP1 acts in homologous recombination (HR) repair, impacts olaparib response, and exhibits aberrant patterns in subsets of human ovarian …

Web8. jan 2024 · High TopBP1 mRNA and protein expression is associated with endometrial cancer. Studies identify the SIRT1-TopBP1 axis as a key signaling mode in the regulation … hello kitty tea setWeb11. nov 2024 · Together, these data hinted that CIP2A collaborates with MDC1 and TOPBP1 to promote the accurate segregation of damaged chromosomes. In support of this … hello kitty television setWeb25. jún 2024 · The authors found that TOPBP1 forms foci in nucleoli of human and mouse cells, which persist during early and mid-S phase, when replication of ribosomal DNA … hello kitty tea shopWebwhich TopBP1 colocalized with 53BP1, had a 2N (G1) DNA content, whereas cells, in which the TopBP1 foci were distinct from the 53BP1 foci, had a DNA content greater than 2N (S- or G2-phases of the cell cycle; Figure 1A–C; Supplementary Figure 2A). In a second approach to explore for cell cycle dependency, we repeated the immunoﬂuores- hello kitty templateWeb21. mar 2024 · TOPBP1 (DNA Topoisomerase II Binding Protein 1) is a Protein Coding gene. ... Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation. Localizes to sites of DNA damage in a H2AX- independent manner. hello kitty teen partyWeb5. okt 2024 · By contrast, we did not detect any foci in blue-light exposed cells 116 expressing the opto-module (mCherry-Cry2) alone, nor in cells expressing the checkpoint clamp 117 subunit opto-RAD9 (RAD9-mCherry-Cry2) (Suppl. Figure 1B). We compared cells expressing 118 WT or W1145R opto-TopBP1. This mutation locates inside TopBP1 ATR … hello kitty testo siglaWebCombined TOPBP1 immunostaining and FISH for rDNA repeats corroborated these results; large nucleolar TOPBP1 foci were covered with dot-like rDNA FISH signals (Fig. 1 E). SIM studies revealed that in most APH-treated HeLa cells, TOPBP1 formed one large focus inside the nucleolus (Fig. 1 F) that corresponded to the large FC, as evidenced by ... hello kitty telefon