Mouse podocyte flow cytometry
NettetNext, Annexin V-FITC/propidium iodide (PI) staining was performed to quantify the podocyte apoptosis via flow cytometry assay. As shown in Figure 5D and E , the rate of apoptotic cells treated with high glucose was significantly increased than control, and the effects of the high glucose treatment were inhibited by curcumin. Nettet10. mai 2024 · In this study, we cultured immortalized mouse podocytes in vitro in different groups: control group; TGF-β1 (5ng/ml) group; EP2 agonist Butaprost treatment (10−7, 10−6, or 10-5mol/L) +TGF-β1 group; EP2 antagonist AH6809 treatment ... In addition, flow cytometry showed increased podocyte apoptosis.
Mouse podocyte flow cytometry
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NettetMouse podocytes were treated with PGE2 (0, 1, 10, 100 nM) for 24 h to induce cell apoptosis. a Representative images of flow cytometry analysis of podocyte apoptosis. b Quantitative analysis of ... Nettet1. okt. 2024 · Idiopathic membranous nephropathy (iMN) is considered an immune-mediated disease where circulating autoantibodies against podocyte targets (mainly …
NettetThe kidneys contain very few lymphocytes under homeostatic conditions. One kidney from a healthy mouse per average contains only 1–5 × 10 3 CD4 + T cells. In immune-mediated kidney disease, γδ T cells, NKT cells, CD4 + T cells, CD8 + T cells, and regulatory T cells (Treg) infiltrate the kidney. Their numbers and subset composition of infiltrating T cells … NettetThis application protocol describes the flow cytometric analysis of macrophages after spleen dissociation from healthy C57BL/6 mice. Viable single cells from mouse spleens are easily obtained using the gentleMACS™ Technology. For downstream flow cytometric analysis of macrophages, we have designed a validated multicolor flow …
NettetIn this study, we have developed a novel method of rapid isolation of podocytes from mice using magnetic activated cell sorting with an anti-nephrin antibody. Methods: After endothelial cell depletion using anti-CD31 antibody, nephrin-positive cells were … Nettet2. nov. 2015 · Flow cytometry is an optical, laser-based technology which analyzes the physical and fluorescent properties of cells in suspension in real-time as they flow …
Nettet9. apr. 2024 · PROTOCOL APRIL 9, 2024 Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry. Zhaoyuan Liu, 1,4,5,* Yaqi Gu, 1,4 Amanda Shin, 1 Shuangyan Zhang, 1 and Florent Ginhoux 1,2,3,6,** 1 Shanghai Institute of Immunology, Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, …
Nettet Flow cytometric analysis of GLUT transporters in control mouse podocytes. Cells were stained for surface content of GLUT1, GLUT2, and GLUT4, as described in Materials … beauty diary yangonNettetUp-regulated β-arrestin 1/2 promoted podocyte apoptosis and p53 pathway by increasing Bax, cleaved caspase-3, and p-p53 levels in high-glucose-induced podocytes. Flow cytometry showed that the ... diners gotovina na rateNettet9. apr. 2024 · Original flow cytometry plots and gating raw data for ... data validate recent findings that mouse is not a suitable model to study MN caused by circulating PLA2R1 antibodies as mouse podocytes ... beauty dilekNettetFlow cytometry was used to detect the effect of β-arrestin 1/2 on apoptosis. (A) Podocytes were stained with FITV A/PE-A for flow cytometry analysis. Apoptotic cells in β-arrestin 1 group were divided into 2 stages (early apoptotic and late apoptotic cells). diners kartica aktivacijaNettetpositive podocytes with I-PpoⅠtransfection and controls, assessed by flow cytometry. (F) Quantification of MHC class Ⅰand Ⅱpositive podocytes with I-PpoⅠ transfection and controls, after 72hr co-cultured with OT-ⅠCD8+ cells, assessed by flow cytometry. diners kartica plaćanje u inozemstvuNettet24. jun. 2024 · Flow cytometric approaches are limited to classifications of either different MNP subsets or functional state. We sought to combine these two … beauty diary makeupNettetI would add CD115. I mice Ly6C + cells represent the majority of circulating monocytes . I would gate on total leukocytes using CD45 then gate on CD115+ cells. The neutrophils should be in your ... beauty diary shop in yangon