WebThe LightCycler ® 480 System features a versatile, plate-based, real-time PCR device that supports mono- or multicolor applications. The benchtop instrument is easily … WebCompact and easy to use, the LightCycler ® 96 System Primary is the ideal companion for applications including gene expression analysis, gene detection, mutation detection, methylation analysis, miRNA research, and relative quantification of target genes. Have confidence in the data you generate and quickly get publication-ready results
LightCycler ® 480 Probes Master - Roche
Webplate: a) LightCycler® 480 Instrument; (b) another real-time PCR instrument. A low target concentration (100 copies) of a given target sequence (442 bp) was amplified using a fast PCR protocol (20 µl reaction volume, hydrolysis probe format). C q values were plotted for all 96 wells using a 0.5-step C q scale resolution. WebEnzymatic hydrolysis experiments (using biotinylated β-galactosidase) of the galacto derivatives have been performed and kinetic parameters were calculated. A 15-20 times increase of the fluorescence intensity has been observed during the hydrolysis. ... making these compounds useful and sensitive probes for galactosidases. The magnitude of ... how does a car starter work
LightCycler ® 480 RNA Master Hydrolysis Probes
WebApr 1, 2024 · qPCR Hydrolysis probe ... Using a modern thermocycling instrument such as the Roche LightCycler 96 (as used in this study), each PCR cycle requires 42 s to heat and cool the reaction. RPA is completed at a constant 37°C without thermal cycling, such that the amplification occurred continuously throughout the incubation, and this contributed to ... WebSep 8, 2005 · To this end, we evaluated the usage of hydrolysis probes using the ABI PRISM 7000 Sequence Detection System (Applied Biosystems, Foster City, USA) and hybridization probes in the LightCycler ... WebHybridization probes (HybProbe format) Hydrolysis probes (“TaqMan” format) Note: Other assay formats may also be adapted for real-time PCR or used in the LightCycler Instrument. For example, adaptable probe formats include Bi-Probes (iFRET-Probes), Molecular Beacons and ScorpionsTM. These will not be described in this Technical Note. 2. phononic inc. ceo